Plasmid Preparation:
Article Title: miRNA-338-3p suppresses cell growth of human colorectal carcinoma by targeting smoothened
Article Snippet: The sequences of interest were inserted into the transfer vector between the Mlu I and Cla I restriction sites according to the Addgene protocol. .. The third generation of self-inactivating, lentivirus plasmid, pLV-THM (HIV-1-based vector; Addgene, Cambridge, MA, United States), which contains a CMV-driven enhanced green fluorescence protein (GFP) reporter and an H 1 promoter upstream of the restriction sites ( Mlu I and Cla I), was used as the transfer plasmid and was linearized by digesting the vector with the restriction enzymes. ..
Article Title: Construction of lentivirus-based inhibitor of hsa-microRNA-338-3p with specific secondary structure
Article Snippet: .. Transfer vector construction The third generation of self-inactivating, lentivirus plasmid, pLV-THM (HIV-1-based vector, Addgene, Cambridge, MA, USA), which contains a CMV-driven enhanced green fluorescence protein (eGFP) reporter and an H 1 promoter upstream of the restriction sites ( Mlu I and Cla I) to allow for the introduction of a short-hairpin RNA (shRNA) expression cassette, was used as the transfer plasmid and was linearized by digesting the vector with the restriction enzymes. .. The miR-338-3p inhibitor oligonucleotides described above were chemically synthesized by Sangon Biotech Co Ltd (Shanghai, China) and were inserted between the Mlu I and Cla I sites of the pLV-THM plasmid.
Article Title: Construction of lentivirus-based inhibitor of hsa-microRNA-338-3p with specific secondary structure
Article Snippet: .. The third generation of self-inactivating, lentivirus plasmid, pLV-THM (HIV-1-based vector, Addgene, Cambridge, MA, USA), which contains a CMV-driven enhanced green fluorescence protein (eGFP) reporter and an H 1 promoter upstream of the restriction sites ( Mlu I and Cla I) to allow for the introduction of a short-hairpin RNA (shRNA) expression cassette, was used as the transfer plasmid and was linearized by digesting the vector with the restriction enzymes. .. The miR-338-3p inhibitor oligonucleotides described above were chemically synthesized by Sangon Biotech Co Ltd (Shanghai, China) and were inserted between the Mlu I and Cla I sites of the pLV-THM plasmid.
Fluorescence:Article Title: miRNA-338-3p suppresses cell growth of human colorectal carcinoma by targeting smoothened
Article Snippet: The sequences of interest were inserted into the transfer vector between the Mlu I and Cla I restriction sites according to the Addgene protocol. .. The third generation of self-inactivating, lentivirus plasmid, pLV-THM (HIV-1-based vector; Addgene, Cambridge, MA, United States), which contains a CMV-driven enhanced green fluorescence protein (GFP) reporter and an H 1 promoter upstream of the restriction sites ( Mlu I and Cla I), was used as the transfer plasmid and was linearized by digesting the vector with the restriction enzymes. ..
Article Title: Construction of lentivirus-based inhibitor of hsa-microRNA-338-3p with specific secondary structure
Article Snippet: .. Transfer vector construction The third generation of self-inactivating, lentivirus plasmid, pLV-THM (HIV-1-based vector, Addgene, Cambridge, MA, USA), which contains a CMV-driven enhanced green fluorescence protein (eGFP) reporter and an H 1 promoter upstream of the restriction sites ( Mlu I and Cla I) to allow for the introduction of a short-hairpin RNA (shRNA) expression cassette, was used as the transfer plasmid and was linearized by digesting the vector with the restriction enzymes. .. The miR-338-3p inhibitor oligonucleotides described above were chemically synthesized by Sangon Biotech Co Ltd (Shanghai, China) and were inserted between the Mlu I and Cla I sites of the pLV-THM plasmid.
Article Title: Construction of lentivirus-based inhibitor of hsa-microRNA-338-3p with specific secondary structure
Article Snippet: .. The third generation of self-inactivating, lentivirus plasmid, pLV-THM (HIV-1-based vector, Addgene, Cambridge, MA, USA), which contains a CMV-driven enhanced green fluorescence protein (eGFP) reporter and an H 1 promoter upstream of the restriction sites ( Mlu I and Cla I) to allow for the introduction of a short-hairpin RNA (shRNA) expression cassette, was used as the transfer plasmid and was linearized by digesting the vector with the restriction enzymes. .. The miR-338-3p inhibitor oligonucleotides described above were chemically synthesized by Sangon Biotech Co Ltd (Shanghai, China) and were inserted between the Mlu I and Cla I sites of the pLV-THM plasmid.
shRNA:Article Title: Construction of lentivirus-based inhibitor of hsa-microRNA-338-3p with specific secondary structure
Article Snippet: .. Transfer vector construction The third generation of self-inactivating, lentivirus plasmid, pLV-THM (HIV-1-based vector, Addgene, Cambridge, MA, USA), which contains a CMV-driven enhanced green fluorescence protein (eGFP) reporter and an H 1 promoter upstream of the restriction sites ( Mlu I and Cla I) to allow for the introduction of a short-hairpin RNA (shRNA) expression cassette, was used as the transfer plasmid and was linearized by digesting the vector with the restriction enzymes. .. The miR-338-3p inhibitor oligonucleotides described above were chemically synthesized by Sangon Biotech Co Ltd (Shanghai, China) and were inserted between the Mlu I and Cla I sites of the pLV-THM plasmid.
Article Title: Construction of lentivirus-based inhibitor of hsa-microRNA-338-3p with specific secondary structure
Article Snippet: .. The third generation of self-inactivating, lentivirus plasmid, pLV-THM (HIV-1-based vector, Addgene, Cambridge, MA, USA), which contains a CMV-driven enhanced green fluorescence protein (eGFP) reporter and an H 1 promoter upstream of the restriction sites ( Mlu I and Cla I) to allow for the introduction of a short-hairpin RNA (shRNA) expression cassette, was used as the transfer plasmid and was linearized by digesting the vector with the restriction enzymes. .. The miR-338-3p inhibitor oligonucleotides described above were chemically synthesized by Sangon Biotech Co Ltd (Shanghai, China) and were inserted between the Mlu I and Cla I sites of the pLV-THM plasmid.
Expressing:Article Title: Construction of lentivirus-based inhibitor of hsa-microRNA-338-3p with specific secondary structure
Article Snippet: .. Transfer vector construction The third generation of self-inactivating, lentivirus plasmid, pLV-THM (HIV-1-based vector, Addgene, Cambridge, MA, USA), which contains a CMV-driven enhanced green fluorescence protein (eGFP) reporter and an H 1 promoter upstream of the restriction sites ( Mlu I and Cla I) to allow for the introduction of a short-hairpin RNA (shRNA) expression cassette, was used as the transfer plasmid and was linearized by digesting the vector with the restriction enzymes. .. The miR-338-3p inhibitor oligonucleotides described above were chemically synthesized by Sangon Biotech Co Ltd (Shanghai, China) and were inserted between the Mlu I and Cla I sites of the pLV-THM plasmid.
Article Title: Construction of lentivirus-based inhibitor of hsa-microRNA-338-3p with specific secondary structure
Article Snippet: .. The third generation of self-inactivating, lentivirus plasmid, pLV-THM (HIV-1-based vector, Addgene, Cambridge, MA, USA), which contains a CMV-driven enhanced green fluorescence protein (eGFP) reporter and an H 1 promoter upstream of the restriction sites ( Mlu I and Cla I) to allow for the introduction of a short-hairpin RNA (shRNA) expression cassette, was used as the transfer plasmid and was linearized by digesting the vector with the restriction enzymes. .. The miR-338-3p inhibitor oligonucleotides described above were chemically synthesized by Sangon Biotech Co Ltd (Shanghai, China) and were inserted between the Mlu I and Cla I sites of the pLV-THM plasmid.
|
Addgene inc is a verified supplier 
